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| Registros recuperados: 18 | |
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| Fechner, Lise C.; Versace, Francois; Gourlay-france, Catherine; Tusseau-vuillemin, Marie-helene. |
| The Water Framework Directive requires the development of biological tools which can act as early-warning indicators of a sudden increase (accidental pollution) or decrease (recovery due to prevention) of the chemical status of aquatic systems. River biofilms, which respond quickly to modifications of environmental parameters and also play a key part in the functioning of aquatic ecosystems, are therefore good candidates to monitor an increase or a decrease of water pollution. In the present study, we investigated the biological response of biofilms transplanted either upstream (recovery) or downstream (deterioration of exposure levels) the urban area of Paris (France). Both modifications of Cu community tolerance levels and of global bacterial and... |
| Tipo: Text |
Palavras-chave: River; Biofilm; PICT; ARISA; Urban contamination; Transplantation. |
| Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00070/18129/16053.pdf |
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| Bourgeault, Adeline; Gourlay-france, Catherine; Priadi, Cindy; Ayrault, Sophie; Tusseau-vuillemin, Marie-helene. |
| This study investigates the ability of the biodynamic model to predict the trophic bioaccumulation of cadmium (Cd), chromium (Cr), copper (Cu), nickel (Ni) and zinc (Zn) in a freshwater bivalve. Zebra mussels were transplanted to three sites along the Seine River (France) and collected monthly for 11 months. Measurements of the metal body burdens in mussels were compared with the predictions from the biodynamic model. The exchangeable fraction of metal particles did not account for the bioavailability of particulate metals, since it did not capture the differences between sites. The assimilation efficiency (AE) parameter is necessary to take into account biotic factors influencing particulate metal bioavailability. The biodynamic model, applied with AEs... |
| Tipo: Text |
Palavras-chave: Bioaccumulation; Active biomonitoring; Bioavailability of particulate metals; Assimilation efficiency; Chemical sequential extraction. |
| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00051/16218/14040.pdf |
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| Chatel, Amelie; Faucet-marquis, Virginie; Pfohl-leszkowicz, Annie; Gourlay-france, Catherine; Vincent Hubert, Francoise. |
| Derived polycyclic aromatic hydrocarbons (PAHs) such as nitro-PAHs are present in the environment and are known to be much more toxic than PAHs compounds. However, very few studies have analysed their effects on the aquatic environment and none have investigated the freshwater environment. In the present study, we determined whether 1-nitropyrene (1-NP), a model of nitro-PAHs, can induce DNA adducts in gills and digestive glands of the freshwater mussel Dreissena polymorpha. Two concentrations of 1-NP (50 and 500 mu M) were tested. In addition, in order to understand the metabolic pathways involved in 1-NP genotoxicity, mRNA expression of genes implicated in biotransformation mechanisms was assessed by quantitative reverse transcription-PCR. Results showed... |
| Tipo: Text |
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| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00287/39799/41532.pdf |
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| Lebrun, Jeremie D.; Uher, Emmanuelle; Tusseau-vuillemin, Marie-helene; Gourlay-france, Catherine. |
| Biomonitoring, assumed to be an integrative measurement of the chemical exposure of aquatic organisms, is not straightforward for essential metals because they can be actively regulated by animals. Although increasing bioaccumulation with exposure levels is a crucial endpoint for the development of biomonitors, it is rarely verified in real environments, where the metal concentrations are rather low and vary little. This study was designed at the scale of a river basin to assess the ability of Gammarus pulex indigenous populations to accumulate Cu, Zn and Mn in realistic exposure conditions. During two annual campaigns, water and gammarids were collected at various sites contrasted in terms of physicochemistry and contamination. The results show... |
| Tipo: Text |
Palavras-chave: Biomonitor; Metal bioavailability; Gammarus pulex; Copper; Zinc; Manganese. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00180/29154/28656.pdf |
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| Chatel, Annelle; Faucet-marquis, Virginie; Gourlay-france, Catherine; Pfohl-leszkowicz, Annie; Hubert, Francoise. |
| The aim of the present study was to confirm the relevance of studying DNA adduct formation in a field study. In that context, freshwater mussels Dreissena polymorpha, collected in a reference station, were transplanted in different sites with a pollution gradient. After one and two months, mussels were collected and DNA adduct formation was analyzed using the 32P post labelling technique on both gills and digestive glands. In addition, the expression of genes involved in the detoxification system (catalase (CAT), superoxide dismutase (SOD), glutathione S-transferase (GST), HSP70, aryl hydrocarbon receptor (AHR), P glycoprotein (PgP), metallothionein (MT)) was assessed by RT-PCR. DNA adducts were observed at amount comparable to data from literature.... |
| Tipo: Text |
Palavras-chave: Biomarker; Pollution; DNA adducts; Detoxification; Dreissena polymorpha; RT-PCR. |
| Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00195/30639/32450.pdf |
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| Chatel, Amelie; Faucet-marquis, Virginie; Perret, Marine; Gourlay-france, Catherine; Uher, Emmanuelle; Pfohl-leszkowicz, Annie; Vincent-hubert, Francoise. |
| The use of DNA adduct analysis has previously focused on the use of marine organisms for biomonitoring, whereas similar investigations in freshwater organisms are sparse. In that context, we have investigated the relevance of DNA adducts as biomarkers of genotoxicity in the freshwater mussels Dreissena polymorpha. The objective of the present study is to determine the stability of DNA adducts induced by benzo[a]pyrene (B[a]P) in zebra mussels. Mussels were exposed to dissolved B[a]P (10100 mu g/l) for 4 days. Afterwards, mussels were kept in clean water for 28 days and DNA adduct levels were subsequently measured in two different organs, the digestive glands and the gills, using the P-32-postlabelling technique. In parallel, the expression of genes... |
| Tipo: Text |
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| Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00184/29566/27912.pdf |
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| Uher, Emmanuelle; Compere, Chantal; Combe, Matthieu; Mazeas, Florence; Gourlay-france, Catherine. |
| Concerning in situ passive sampler deployment, several technical priorities must be considered. In particular, deployment time must be sufficiently long not only to allow a significant quantity to be accumulated to facilitate analysis but also to ensure that the signal is above the quantification limit and out of the blank influence. Moreover, regarding the diffusive gradient in thin films (DGT) technique, deployment time must also be sufficiently long (at least 5 days) to avoid the interactions of the solutes with the material diffusion layer of the DGT and for the steady state to be reached in the gel. However, biofouling occurs in situ and modifies the surface of the samplers. In this article, we propose a kinetic model which highlights the biofouling... |
| Tipo: Text |
Palavras-chave: DGT; Metals; Biofouling; Seine River; Field deployment; Passive sampler. |
| Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00382/49330/49759.pdf |
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| Fechner, Lise C.; Gourlay-france, Catherine; Tusseau-vuillemin, Marie-helene. |
| It is difficult to assess the biological consequences of diffuse water contamination by micropollutants which are present in rivers at low, even sublethal levels. River biofilms, which respond quickly to changes of environmental parameters, are good candidates to acquire knowledge on the response of aquatic organisms to diffuse chemical contamination in the field. The study was designed as an attempt to link biofilm metal tolerance and metallic contamination in a field survey covering 13 different sampling sites in the Seine river basin (north of France) with low contamination levels. Cd and Zn tolerance of heterotrophic communities was assessed using a short-term toxicity test based on β-glucosidase activity. Metal tolerance levels varied between sites... |
| Tipo: Text |
Palavras-chave: River biofilm; Metal tolerance; Toxicity; ARISA; PICT; Community-level response. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00169/28072/26287.pdf |
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| Fechner, Lise C.; Gourlay-france, Catherine; Tusseau-vuillemin, Marie-helene. |
| The biological response of periphyton chronically exposed to metals of urban origin (Cd, Ni and Zn) was investigated with a Pollution-Induced Community Tolerance (PICT) approach using a previously developed short-term toxicity test based on beta-glucosidase (heterotrophic) activity. Periphyton was grown on plastic membranes immersed in indoor aquaria contaminated with metals at realistic contamination levels (0.3, 3 mu g/l for Cd, 5, 50 mu g/l for Ni, 20, 200 mu g/l for Zn). After 3 weeks of exposure, biofilms' parameters (dry-weight, chlorophyll a concentration, heterotrophic activity) were analyzed and tolerance acquisition of the heterotrophic communities was assessed using the toxicity test. Modifications of bacterial and eukaryotic community structure... |
| Tipo: Text |
Palavras-chave: Biofilms; Urban metal; Community tolerance; ARISA. |
| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00036/14682/12028.pdf |
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| Vincent-hubert, Francoise; Chatel, Amelie; Gourlay-france, Catherine. |
| We have previously shown that cadmium (Cd) and Benzo[a]pyrene (BaP) induced early DNA damages in zebra mussels, and that the level of DNA strand breaks (SB) returned to a basal level after 3 days of exposure to Cd. The aim of the present study was to go further in the mechanisms of Cd and BaP detoxification. For that purpose, expression of genes encoding for metallothionein (MT), Aryl Hydrocarbon Receptor (AHR), P-gP, catalase, glutathione S-transferase and Heat shock protein 70 (HSP70) proteins have been measured using RT-qPCR. Data reported here show that Cd is a strong inducer of MT and HSP70 genes, and that BaP is a strong inducer of P-gP and AHR genes. Exposure to Cd and BaP resulted in moderate changes in antioxidant enzymes mRNA. Since the increase... |
| Tipo: Text |
Palavras-chave: Benzo[a]pyrene; Cadmium; Metallothionein; Aryl hydrocarbon receptor; Antioxidant enzymes; RT-qPCR. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00184/29547/27873.pdf |
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| Pellet, Bastien; Ayrault, Sophie; Tusseau-vuillemin, Marie-helene; Gourlay-france, Catherine. |
| Gammarids are aquatic amphipods widely used for water quality monitoring. To investigate the copper and cadmium diet-borne metal uptake in Gammarus pulex, we adapted the pulse-chase stable isotopes-based approach to determine the food ingestion rate (IR), the gut retention time (GRT) and the metal assimilation efficiencies (AE). G. pulex were fed with 65Cu-, 106Cd-, and 53Cr-labeled alder leaves for 7.5 h and then with unlabeled leaves for 5 d. The metal stable isotope contents in the gammarids, leaves, filtered water and periodically collected feces were determined. Chromium was poorly assimilated by the gammarids; thus, Cr was used as an unassimilated tracer. The first tracer defecation occurred before the first feces harvest, indicating a gut passage... |
| Tipo: Text |
Palavras-chave: Assimilation efficiency; Gammarus pulex; Cadmium; Copper; Biodynamic model; Diet-borne uptake. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00211/32238/31762.pdf |
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| Gourlay-france, Catherine; Delmas, François; Mazzella, Nicolas; Tusseau-vuillemin, Marie-helene. |
| La biodisponibilité d'une substance chimique désigne sa capacité à interagir avec les organismes vivants. Cette notion est très importante dans l’évaluation du risque écotoxicologique dont la pertinence repose notamment sur l'aptitude à prédire quelle sera la fraction contaminante des micro-polluants susceptible d'exercer une action toxique sur les organismes. Plusieurs méthodes chimique et/ou biologique, sont proposées pour estimer la biodisponiblité des susbtances, qui dépend à la fois des caractéristiques physico-chimiques du milieu et de la physiologie des organismes exposés. Néanmoins, que savons-nous de la biodisponibilité des contaminants et sur ce qui l'influence dans le milieu aquatique ? Cet article fait le point sur ce nouveau champ d'étude... |
| Tipo: Text |
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| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00068/17974/15482.pdf |
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| Priadi, Cindy; Bourgeault, Adeline; Ayrault, Sophie; Gourlay-france, Catherine; Tusseau-vuillemin, Marie-helene; Bonte, Philippe; Mouchel, Jean-marie. |
| In order to obtain representative dissolved and solid samples from the aquatic environment, a spectrum of sampling methods are available, each one with different advantages and drawbacks. This article evaluates the use of discrete sampling and time-integrated sampling in illustrating medium-term spatial and temporal variation. Discrete concentration index (CI) calculated as the ratio between dissolved and solid metal concentrations in grab samples are compared with time-integrated concentration index (CI) calculated from suspended particulate matter (SPM) collected in sediment traps and labile metals measured by the diffusive gel in thin films (DGT) method, collected once a month during one year at the Seine River, upstream and downstream of the Greater... |
| Tipo: Text |
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| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00036/14678/12111.pdf |
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| Lebrun, Jeremie D.; Dufour, Marine; Uher, Emmanuelle; Fabure, Juliette; Mons, Raphael; Charlatchka, Rayna; Gourlay-france, Catherine; Fechner, Lise; Ferrari, Benoit Jd. |
| Dredging generates remobilisation of sediments contaminated by non-degradable compounds such as metals, to which aquatic organisms can be exposed. This study aims at assessing the environmental impact of sediments remobilised in the Rhine River (France) during the dredging of Marckolsheim dam by pumping/dilution in 2013 on metal speciation and organisms' exposure. The monitoring coupling chemical and biological tools was performed 2 years before dredging operation on 2 sampling sites, upstream and downstream from the discharge of pumping/dilution, to acquire data on the natural variability of labile (DGT as passive samplers), dissolved and particulate concentrations of Cd, Co, Cr, Cu, Ni, Mn, Pb and Zn in Rhine during full hydrological cycles. In parallel,... |
| Tipo: Text |
Palavras-chave: Active biomonitoring; Dreissena polymorpha; DGT; Metal bioavailability; Natural variability. |
| Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00413/52458/53244.pdf |
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| Registros recuperados: 18 | |
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